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KMID : 1134820070360020155
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Journal of the Korean Society of Food Science and Nutrition
2007 Volume.36 No. 2 p.155 ~ p.162
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Inhibitory Effects of Phyto-Extract Mixture (PEM381) on Type Ⅰ Allergic Reaction
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Kim Kyung-Bum
Lee Eu-Gene
Chai Ok-Hee
Song Chang-Ho
Jeong Jong-Moon
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Abstract
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The purpose of this study is to investigate the anti-type Ⅰ allergic effects and mechanisms of the phyto-extract mixture (PEM381) which contains Camellia sinensis (leaf), Psidium guajava (leaf), and Rosa hybrida(flower). PEM381 was tested for its inhibitory effects on arachidonic acid cascade-related enzymes (5-lipoxygenase and cyclooxygenase), the mast cell-mediated allergic reaction and passive cutaneous anaphylaxis. IC50 value of PEM381 against 5-lipoxygenase was 14.11±0.51 ppm while that of positive control (nordihy-droguaiaretic acid) was 0.54±0.08 ppm. PEM381 also exhibited considerable selective inhibition of cy-clooxygenase-2. PEM381 could inhibit both degranulation and histamine release in a dose-dependent manner from rat peritoneal mast cells activated by compound 48/80. In addition, oral administration of PEM381 showed an inhibitory effect on passive cutaneous anaphylaxis reaction activated by anti-dinitrophenyl IgE antibody in mice. These results suggest that PEM381 may be useful for the prevention and treatment of type Ⅰ allergy-related diseases.
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KEYWORD
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PEM381, 5-lipoxygenase, cyclooxygenase, mast cell, histamine
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